Download MendeleyStructure of Thermotoga maritima GH5 endoglucanase TM1752 in complex with TRIS
Manoj, N., Garg, P.To be published.
7EFZ
Structure of Thermotoga maritima GH5 endoglucanase TM1752 in complex with TRIS
- PDB DOI: https://doi.org/10.2210/pdb7EFZ/pdb
- Classification: HYDROLASE
- Organism(s): Thermotoga maritima MSB8
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2021-03-23 Released: 2022-03-30
- Funding Organization(s): Department of Biotechnology (DBT, India)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.69 Å
- R-Value Free: 0.186
- R-Value Work: 0.155
- R-Value Observed: 0.156
Starting Model: experimental
View more details
This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Endoglucanase | 341 | Thermotoga maritima MSB8 | Mutation(s): 0 Gene Names: TM_1752 |  | |
UniProt | |||||
Find proteins for Q9X274 (Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)) Explore Q9X274 Go to UniProtKB: Q9X274 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9X274 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| TRS (Subject of Investigation/LOI) Query on TRS | C [auth A], E [auth B] | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL C4 H12 N O3 LENZDBCJOHFCAS-UHFFFAOYSA-O |  | ||
| IPA Query on IPA | D [auth A], F [auth B], G [auth B] | ISOPROPYL ALCOHOL C3 H8 O KFZMGEQAYNKOFK-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.69 Å
- R-Value Free: 0.186
- R-Value Work: 0.155
- R-Value Observed: 0.156
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 61 | α = 90 |
| b = 61.61 | β = 90 |
| c = 201.68 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| MOSFLM | data reduction |
| Aimless | data scaling |
| PDB_EXTRACT | data extraction |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
| Funding Organization | Location | Grant Number |
|---|---|---|
| Department of Biotechnology (DBT, India) | India | BT/PR-13040/FNS/20/416/2009 |
Revision History (Full details and data files)
- Version 1.0: 2022-03-30
Type: Initial release - Version 1.1: 2023-11-29
Changes: Data collection, Refinement description
