8X7F

CryoEM structure of the trifunctional NAD biosynthesis/regulator protein NadR in complex with NAD

PDB DOI: https://doi.org/10.2210/pdb8X7F/pdb
EM Map EMD-38098: EMDB EMDataResource

Classification: CELL INVASION
Organism(s): Escherichia coli K-12
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2023-11-24 Released: 2024-12-04
Deposition Author(s): Jiang, W.X., Dong, X., Cheng, X.Q., Ma, L.X., Xing, Q.
Funding Organization(s): National Basic Research Program of China (973 Program)

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 2.66 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

CryoEM structure of the trifunctional NAD biosynthesis/regulator protein NadR in complex with NAD

Jiang, W.X., Dong, X., Cheng, X.Q., Ma, L.X., Xing, Q.

To be published.

Macromolecule Content

Total Structure Weight: 164.61 kDa
Atom Count: 11,596
Modelled Residue Count: 1,388
Deposited Residue Count: 1,388
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Trifunctional NAD biosynthesis/regulator protein NadR	A, B [auth D], C [auth G], D [auth J]	347	Escherichia coli K-12	Mutation(s): 0 Gene Names: nadR, nadI, b4390, JW5800 EC: 2.7.7.1 (PDB Primary Data), 2.7.1.22 (PDB Primary Data)
UniProt
Find proteins for P27278 (Escherichia coli (strain K12)) Explore P27278 Go to UniProtKB: P27278
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P27278
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 2 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
NAD (Subject of Investigation/LOI) Query on NAD Download Ideal Coordinates CCD File SDF format, chain E [auth A] SDF format, chain G [auth D] SDF format, chain I [auth G] SDF format, chain K [auth J] MOL2 format, chain E [auth A] MOL2 format, chain G [auth D] MOL2 format, chain I [auth G] MOL2 format, chain K [auth J] mmCIF format, chain E [auth A] mmCIF format, chain G [auth D] mmCIF format, chain I [auth G] mmCIF format, chain K [auth J]	E [auth A], G [auth D], I [auth G], K [auth J]	NICOTINAMIDE-ADENINE-DINUCLEOTIDE C₂₁ H₂₇ N₇ O₁₄ P₂ BAWFJGJZGIEFAR-NNYOXOHSSA-N		Interactions Focus chain E [auth A] Focus chain G [auth D] Focus chain I [auth G] Focus chain K [auth J] Interactions & Density Focus chain E [auth A] Focus chain G [auth D] Focus chain I [auth G] Focus chain K [auth J]
PO4 (Subject of Investigation/LOI) Query on PO4 Download Ideal Coordinates CCD File SDF format, chain F [auth A] SDF format, chain H [auth D] SDF format, chain J [auth G] SDF format, chain L [auth J] MOL2 format, chain F [auth A] MOL2 format, chain H [auth D] MOL2 format, chain J [auth G] MOL2 format, chain L [auth J] mmCIF format, chain F [auth A] mmCIF format, chain H [auth D] mmCIF format, chain J [auth G] mmCIF format, chain L [auth J]	F [auth A], H [auth D], J [auth G], L [auth J]	PHOSPHATE ION O₄ P NBIIXXVUZAFLBC-UHFFFAOYSA-K		Interactions Focus chain F [auth A] Focus chain H [auth D] Focus chain J [auth G] Focus chain L [auth J] Interactions & Density Focus chain F [auth A] Focus chain H [auth D] Focus chain J [auth G] Focus chain L [auth J]