Download MendeleyRewiring Catalytic Craters: A Path for Engineering beta-Glucosidases to Improve Glucose Tolerance
Suryawanshi, A.B., Bedi, R.K., Pawar, M., Noronha, S., Bhaumik, P.(2025) bioRxiv
9JLZ | pdb_00009jlz
High resolution crystal structure of GH1 beta-glucosidase from soil metagenome (UnBGl1)
- PDB DOI: https://doi.org/10.2210/pdb9JLZ/pdb
- Classification: HYDROLASE
- Organism(s): soil metagenome
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2024-09-20 Released: 2026-02-04
- Funding Organization(s): Department of Biotechnology (DBT, India)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.15 Å
- R-Value Free: 0.147 (Depositor), 0.148 (DCC)
- R-Value Work: 0.126 (Depositor), 0.127 (DCC)
- R-Value Observed: 0.127 (Depositor)
Starting Model: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Beta-glucosidase | 477 | soil metagenome | Mutation(s): 0 EC: 3.2.1.21 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PO4 Query on PO4 | B [auth A] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
| GOL Query on GOL | E [auth A], F [auth A], G [auth A] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
| CL Query on CL | C [auth A], D [auth A] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.15 Å
- R-Value Free: 0.147 (Depositor), 0.148 (DCC)
- R-Value Work: 0.126 (Depositor), 0.127 (DCC)
- R-Value Observed: 0.127 (Depositor)
Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 51.84 | α = 90 |
| b = 65.99 | β = 90 |
| c = 138.18 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| XDS | data reduction |
| XDS | data scaling |
| MOLREP | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2026-02-04 Deposition Author(s): Suryawanshi, A.B., Bhaumik, P.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Department of Biotechnology (DBT, India) | India | BT/PR41982/PBD/26/822/2021 |
Revision History (Full details and data files)
- Version 1.0: 2026-02-04
Type: Initial release
